Aims and Background: Nowadays, monoclonal antibodies (mAbs) became powerful therapeutic and diagnostic tools. Due to showing minimum immunogenic reaction and their properties, human mAbs are important. The purpose of this study was to construct an immune antibody library from a vaccinated donor against tetanus toxin.Materials and Methods: A whole blood sample was taken from the donor who was vaccinated against tetanus toxoid. PBMC were isolated by using ficol. After RNA extraction, all variation of VH and VL regions by RT-PCR reactions were amplified and linked as a ScFv antibody. The amplicons were inserted in T-VECTOR and transformed to E. coli DH5α strain, followed by an ELISA test. Plasmids were also extracted and sequenced.Results: cDNA quality was confirmed by using HPRT primers. To confirm PCR, insertion and transformation, gel electrophoresis and restriction enzymes digestion were applied. Positive clones were selected based on growth on LB agar which is the blue/white selection method. After plasmid extraction and DNA sequencing, the sequences were aligned using igBLAST at NCBI. The result was shown to have admissible similarity among antibody gene library nucleotide sequences and the antibody genes were deposited in this database. ELISA confirmed this data too.Conclusion: In this study, the immune human antibody library was constructed and confirmed using DNA sequencing and sequences alignment in NCBI database. ELISA test confirms antibody specifically. The next step is to screen the library to find an antibody specifically for tetanus toxin.

A B S T R A C T Considering the location of vital centers in the heart of the cities, they are more affected by unexpected events. Hence, due to the probability of the loss of existing services and devastating effects on the social organization and the necessity to prepare essential needs of people and military agencies, planning and building the storage for essential items as a replacement for the damaged services are considered a critical factor in social and defensive reliability. This research tries to locate stockpile of essential Items in Mashhad by emphasizing passive defense criteria and analyzing stockpiles of essential items in terms of form communicational structure. The type of research is applied, and the research method is in theoretical discussion and formulation of mental models, descriptive-analytical and information-gathering methods, documents, questionnaires, and interviews. Moreover, the information analysis method is with the IHWP model in GIS software in the locating section. In the section on examining architectural patterns and FORMS is content analysis. The research results show that 20.61 percent of Mashhad has an optimal location potential, and 0.94 percent of city also has a favorable potential for location. Also, parts of the north-east and south of the city have the priority for location. The types of spatial organization of the rectangular form, including centralized, four-armed, introverted, and diagonal, were investigated in the architecture department. The centralized form is preferable to other FORMS for its capacity and access to exits and spatial organization Extended Abstract Introduction As a stockpile of essential items to facilitate the process of activities and to achieve goals, the warehouse plays a vital role in creating this flexibility in organizations. In fact, from the point of view of crisis management, the stockpile of essential items can play a significant role in the supply chain to deal with unforeseen internal or external incidents. Passive defense in the stockpile of essential items refers to measures that do not require expensive and challenging methods and solutions to protect the stockpile of essential items. Furthermore, it can help avoid the substantial expenses of monitoring the entire inventory and increasing non-conformities (such as theft, fire, non-conformity in the inventory and management inspection, fatal accidents, etc.) or reduce this amount. Choosing the form of the stockpile of essential items for essential items with a passive defense approach requires a comprehensive analysis based on defense principles such as hierarchical protection, nationwide coverage, dynamic protection, relative self-sufficiency, and minimum vulnerability. Therefore, in addition to placing this category of stockpile of essential items in Mashhad, the mentioned research also tries to establish a relationship between architectural FORMS and principles of defense to reduce vulnerability and improve the level of relief in times of crisis.   Methodology The current type of research is applied, and the research method is descriptive-analytical. In this research, 29 people were interviewed using semi-structured questionnaires and in-depth interviews with urban and passive defense experts. The method of data analysis is quantitative and qualitative. Also, the content analysis method was used to compile and analyze the architectural FORMS of the stockpile of essential items of essential items compatible with the principles of passive defense. The fuzzy quantitative model IHWP was exploited in GIS software for placing the stockpile of essential items in the studied sample.   Results and discussion Locating From the overlaying of 10 indicators, distance from the fault, height of buildings, distance from gas lines, distance from high voltage power lines, road width, building density, risk of uses, distance from water transmission lines, population density, and land prone to liquefaction, location in 10 class was conducted that the first and second priority is the north-east and south of Mashhad, which has the most favorable location. In Mashhad, 17.2% of the city area has a very low score, 12.8% low score, 48.37% medium score, 20.61% high score, and 0.94% very high score regarding location desirability.   Architecture According to the previous studies on geometric FORMS, the suitable form for warehouse design is a simple form that does not have frequent breaks and sharp and vague points to reduce the spatial deviation. Among the geometrical shapes, square and rectangular right-cornered shapes meet the criteria of warehouse design. In the meantime, the rectangle can define joints along its length, each of which is a focal point for concentrating activities and organizing their performance. Additionally, the internal spaces in this form are reasonably defined, which results in the building's overall form being overshadowed. This also makes the surrounding accesses more targeted and focused, ultimately providing more suitable relief during times of crisis. In order to create four types of stockpiles of essential items, A (centralized), B (four arms), C (introverted), and D (polar) were designed, and the way of combining these stockpiles of essential items was expressed and analyzed separately. The research results on accesses and circulation shows that type A covers a population of over 100,000 people, which has the best performance in terms of access to exits and spatial organization and provides services in times of crisis. Therefore, type A, due to the sequence of layers, lines, and expansion on the surface, has the most functional symmetry with the body of the warehouse, and its spatial projection is limited to adapt the shell to the body. Visually, it has the least visibility from a high height. Due to its volume and formal indicators, it has a high level of homogeneity and camouflage with its surrounding environment.   Conclusion The results of placing stockpiles of essential items in Mashhad showed that the northeast and south of the city have a higher talent for establishment. According to the examination of geometric FORMS, the rectangular form has the most efficiency for warehouse design in the architecture area. Due to formal capabilities such as the value of lines, angles, and proportions, it has the most efficiency in terms of performance, motion circulation, and the least spatial deviation. In order to research as mentioned above, after choosing the dominant form of the rectangle, an attempt has been made to analyze the spatial organization of the rectangular form using linear, combined, decomposition, and combination methods and centripetal and radial arrangements in terms of passive defense principles. Furthermore, four different types of warehouse design were formally analyzed as patterns and representative types, which are four arms, centralized, introverted, and diagonal, each of which, in addition to functional capabilities, also covers the population's needs in crisis conditions. Finally, the concentrated type due to the extent of its successive lines and layers in close proximity to each other, the perceptibility of the surfaces of the body joints because it can be recognized from a high altitude in order not to be identified in times of crisis such as air raid, they have the highest degree of compatibility between function and form from the point of view of passive defense.   Funding There is no funding support.   Authors’ Contribution All of the authors approved the content of the manuscript and agreed on all aspects of the work.     Conflict of Interest Authors declared no conflict of interest.   Acknowledgments We are grateful to all the scientific

In this paper, some properties of  pseudoinvex functions, defined by means of  limiting subdifferential, are discussed. Furthermore, the Minty VECTOR variational-like inequality,  the Stampacchia VECTOR variational-like inequality, and the  weak formulations of these two inequalities  defined by means of limiting subdifferential are studied. Moreover, some relationships  between the VECTOR variational-like inequalities and VECTOR optimization problems are established.

We call a local VECTOR lattice any VECTOR lattice with a distinguished positive strong unit and having exactly one maximal ideal (its radical). We provide a short study of local VECTOR lattices. In this regards, some characterizations of local VECTOR lattices are given. For instance, we prove that a VECTOR lattice with a distinguished strong unit is local if and only if it is clean with non no-trivial components. Nevertheless, our main purpose is to prove, via what we call the radical functor, that the category of all VECTOR lattices and lattice homomorphisms is equivalent to the category of local VECTORs lattices and unital (i. e., unit preserving) lattice homomorphisms.

In this article, we examine a nonsmooth VECTOR optimization problem with locally Lipschitz approximately convex mappings in terms of the convexificator and provide some ideas for approximate effective solutions. Additionally, we define the relationship between the convexificator-based solutions of Stampacchia type VECTOR variational inequalities ($VVI$) and the approximate efficient approximation convex function of nonsmooth VECTOR optimization problems using the locally Lipschitz function. Furthermore, we provide a numerical example to demonstrate the veracity of our findings.